CHO-K1/Rat 5-HT1B Stable Cell
Item | Cat# | Price |
Stable Cell Line | SNB-G-0002C | $19,800 |
Compound Testing Services | CT-001 | $1,850 per 384w plate (Up To 16 cpds Dose) |
Product Description
5-HT1B receptor (HTR1B) are expressed throughout the mammalian central nervous system. These receptors are located in the axon terminals of both serotonergic and nonserotonergic neurons, where they act as inhibitory autoreceptors or heteroreceptors, respectively. 5-HT1B receptors inhibit the release of a range of neurotransmitters, including serotonin, GABA, acetylcholine, and glutamate.
Screeningbio’s CHO-K1/Rat 5-HT1B cell line overexpress HTR1B and is designed to detect inhibition in intracellular cAMP levels in response to agonist stimulation of the receptor. Cisbio HTRF cAMP kit can be used to detect the signal.
Product Specifications
Target Type | GPCR |
Species | Rat |
HGNC Symbol | 5-HT1B |
Accession Number | NM_022225 (Rn) |
Parental Line | CHO-K1 |
Lot# | See Vial |
Storage | Liquid Nitrogen |
Data
![CHO-K1/Rat 5-HT1B Antagonist Assay. CHO-K1/ Rat 5-HT1B cells were stimulated with 2.5 μM Forskolin and treated with reference antagonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software.](https://static.wixstatic.com/media/cbf7de_d647526c23704d9e9d2d6ab0d20bb0ec~mv2.png/v1/fill/w_75,h_75,al_c,q_85,usm_0.66_1.00_0.01,blur_2,enc_auto/cbf7de_d647526c23704d9e9d2d6ab0d20bb0ec~mv2.png)
Target Background
5-HT1B receptor (HTR1B) are expressed throughout the mammalian central nervous system. These receptors are located in the axon terminals of both serotonergic and nonserotonergic neurons, where they act as inhibitory autoreceptors or heteroreceptors, respectively. 5-HT1B receptors inhibit the release of a range of neurotransmitters, including serotonin, GABA, acetylcholine, and glutamate.