HEK293 Human 5-HT2A Stable Cell

HEK293/Human 5-HT2A Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0006A	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

The 5-HT2A receptor (5-hydroxytryptamine receptor 2A) is a G protein-coupled receptor widely distributed throughout the body. It is involved in regulating various central physiological functions, such as mood, memory, sleep, nociception, feeding, and reward-related behaviors. In addition, this receptor is also believed to play a role in modulating the cardiovascular system. Studies have shown that depressed patients have a higher number of 5-HT2A receptors compared to healthy individuals, suggesting that an increase in the density of these receptors may be directly linked to the pathogenesis of the disorder.

Screeningbio’s HEK293/Human 5-HT2A cell line overexpress HTR2A receptor and is designed to detect increases in intracellular IP-1 levels in response to agonist stimulation of the receptor. Cisbio HTRF IP-1 kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Human
HGNC Symbol	5-HT2A
Accession Number	NM_000621
Parental Line	HEK293
Lot#	See Vial
Storage	Liquid Nitrogen

Data

HEK293/Human 5-HT2A Agonist Assay. HEK293/Human 5-HT2A cells were treated with the reference agonist. The assay was run based on Revvity IP-one HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software. — **HEK293/Human 5-HT2A Agonist Assay.** HEK293/Human 5-HT2A cells were treated with the reference agonist. The assay was run based on Revvity IP-one HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software.

HEK293/Human 5-HT2A Antagonist Assay. HEK293/Human 5-HT2A cells were treated with the reference antagonist. The assay was run based on Revvity IP-one HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software. — **HEK293/Human 5-HT2A Antagonist Assay.** HEK293/Human 5-HT2A cells were treated with the reference antagonist. The assay was run based on Revvity IP-one HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software.

Target Background

The 5-HT2A receptor (5-hydroxytryptamine receptor 2A) is a G protein-coupled receptor widely distributed throughout the body. It is involved in regulating various central physiological functions, such as mood, memory, sleep, nociception, feeding, and reward-related behaviors. In addition, this receptor is also believed to play a role in modulating the cardiovascular system.

Studies have shown that depressed patients have a higher number of 5-HT2A receptors compared to healthy individuals, suggesting that an increase in the density of these receptors may be directly linked to the pathogenesis of the disorder.