HEK293T Human β1-adrenoceptor β Arrestin Stable Cell
Item | Cat# | Price |
Stable Cell Line | SNB-A-0028A | Inquiry |
Compound Testing Services | CT-001 | $1,850 per 384w plate (Up To 16 cpds Dose) |
Product Description
The β1-adrenoceptor (beta-1 adrenergic receptor) is a G-protein-coupled receptor (GPCR) predominantly located in the heart and kidneys. It is the primary adrenergic receptor subtype in the heart, where its activation by catecholamines (e.g., noradrenaline, adrenaline) stimulates Gs-protein signaling, leading to increased heart rate (chronotropy), enhanced contractile force (inotropy), and accelerated conduction velocity (dromotropy). In the kidneys, β1-receptor activation stimulates the release of renin, thereby activating the renin-angiotensin-aldosterone system (RAAS).Due to its crucial role in cardiac function, β1-adrenoceptors are a major therapeutic target.
ScreeningBio’s HEK293T/Human β1-adrenoceptor β-Arrestin cell line co-express the SmBiT tagged β1-adrenoceptor and the LgBiT tagged β-Arrestin. Activation of the GPCR-SmBiT induces β-Arrestin-LgBiT recruitment, forcing complementation of two NanoBiT enzyme fragments (SmBiT and LgBiT). The resulting functional NanoBiT enzyme catalyze substrate to generate a luminescent signal. Promega Nano-Glo Live Cell Kit can be used to detect the signal.
Product Specifications
Target Type | GPCR |
Species | Human |
HGNC Symbol | ADRB1 |
Accession Number | NM_000684 (Hs) |
Parental Line | HEK293T |
Lot# | See Vial |
Storage | Liquid Nitrogen |
Data
Target Background
The β1-adrenoceptor (beta-1 adrenergic receptor) is a G-protein-coupled receptor (GPCR) predominantly located in the heart and kidneys. It is the primary adrenergic receptor subtype in the heart, where its activation by catecholamines (e.g., noradrenaline, adrenaline) stimulates Gs-protein signaling, leading to increased heart rate (chronotropy), enhanced contractile force (inotropy), and accelerated conduction velocity (dromotropy).
In the kidneys, β1-receptor activation stimulates the release of renin, thereby activating the renin-angiotensin-aldosterone system (RAAS).Due to its crucial role in cardiac function, β1-adrenoceptors are a major therapeutic target.