CHO-K1 Human α2A Stable Cell
Item | Cat# | Price |
Stable Cell Line | SNB-G-0025D | $19,800 |
Compound Testing Services | CT-001 | $1,850 per 384w plate (Up To 16 cpds Dose) |
Product Description
Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily, comprising three highly homologous subtypes: α2A, α2B, and α2C. These receptors play a critical role in regulating neurotransmitter release from sympathetic nerves and adrenergic neurons in the central nervous system. Studies in mice have revealed that both the α2A and α2C subtypes are required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. Specifically, the α2A subtype inhibits transmitter release at high stimulation frequencies, whereas the α2C subtype modulates neurotransmission at lower levels of nerve activity.
Screeningbio’s CHO-K1/Human α2A cell line overexpress human α2A-adrenoceptor and is designed to detect inhibition in intracellular cAMP levels in response to agonist stimulation of the receptor. HTRF cAMP kit can be used to detect the signal.
Product Specifications
Target Type | GPCR |
Species | Human |
HGNC Symbol | ADRA2A |
Accession Number | NM_000681 (Hs) |
Parental Line | CHO-K1 |
Lot# | See Vial |
Storage | Liquid Nitrogen |
Data
![CHO-K1/Human α2A Antagonist Assay. CHO-K1/Human α2A cells were treated with the reference antagonist. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software.](https://static.wixstatic.com/media/cbf7de_0a2eae9e581f4d5d9cfb630fa06b919e~mv2.png/v1/fill/w_75,h_75,al_c,q_85,usm_0.66_1.00_0.01,blur_2,enc_auto/cbf7de_0a2eae9e581f4d5d9cfb630fa06b919e~mv2.png)
Target Background
Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily, comprising three highly homologous subtypes: α2A, α2B, and α2C. These receptors play a critical role in regulating neurotransmitter release from sympathetic nerves and adrenergic neurons in the central nervous system.
Studies in mice have revealed that both the α2A and α2C subtypes are required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. Specifically, the α2A subtype inhibits transmitter release at high stimulation frequencies, whereas the α2C subtype modulates neurotransmission at lower levels of nerve activity.