U2OS NRF2 nuclear translocation Cell Line
Item | Cat# | Price |
Stable Cell Line | SNB-SP-0015 | $1,980 |
Compound Test Services | CT-001 | $1,850 per 384w plate (Up To 16 cpds Dose) |
Product Description
The U2OS NRF2 nuclear translocation cell line is engineered to co-express two proteins: a) Enzyme Donor tagged NRF2; b) an Enzyme Acceptor, which is localized to the nucleus. Depending on the assay, activation of the signaling pathway can either a) induce translocation of the ED-tagged target protein into nucleus, that will force complementation of the two enzyme fragments, and result in the formation of a functional enzyme, that will hydrolyze substrate and generate a chemiluminescent signal; b) induce the ED-tagged NRF2 to vacate the nucleus, resulting in a decrease of functional enzyme and a subsequent decrease of chemiluminescent signal.
ScreeNingBio U2OS NRF2 cell line will also co-express an untagged secondary protein KEAP1.
Product Specifications
Target Type | Signal Pathway |
Species | Human |
HGNC Symbol | |
Accession Number | |
Parental Line | U2OS |
Lot# | See Vial |
Storage | Liquid Nitrogen |
Data
Target Background
The U2OS NRF2 nuclear translocation cell line is engineered to co-express two proteins: a) Enzyme Donor tagged NRF2; b) an Enzyme Acceptor, which is localized to the nucleus. Depending on the assay, activation of the signaling pathway can either a) induce translocation of the ED-tagged target protein into nucleus, that will force complementation of the two enzyme fragments, and result in the formation of a functional enzyme, that will hydrolyze substrate and generate a chemiluminescent signal; b) induce the ED-tagged NRF2 to vacate the nucleus, resulting in a decrease of functional enzyme and a subsequent decrease of chemiluminescent signal.