NIH3T3 Gli-dependent Dual-Luciferase Reporter Cell Line
Item | Cat# | Price |
Stable Cell Line | SNB-SP-0003 | $19,80 |
Compound Test Services | CT-001 | $1,850 per 384w plate (Up To 16 cpds Dose) |
Product Description
The NIH3T3 Gli-dependent Dual Reporter (Luc) cell line stably express a Gli-responsive Firefly luciferase and a constitutively express a Renilla luciferase as an internal control for cell density, it is widely used to study activation and inhibition of canonical Hedgehog signaling. The Sonic Hedgehog (Shh) signaling pathway plays a critical role during embryonic development and cancer progression. Various cancers exhibit active Hedgehog signaling. This Gli-mediated transcriptional luciferase reporter assay can be used to evaluate compounds activation/ inhibition of human Gli-mediated transcription.
Screeningbio’s NIH3T3/Gli-dependent Dual/Luciferase reporter gene cell line stable transfected with Gli response element and luciferase gene. Upon stimulated, Hedgehog pathway was activated and induce luciferase expression.
Product Specifications
Target Type | Signal Pathway |
Species | Human |
HGNC Symbol | |
Accession Number | |
Parental Line | NIH3T3 |
Lot# | See Vial |
Storage | Liquid Nitrogen |
Data
Target Background
The NIH3T3 Gli-dependent Dual Reporter (Luc) cell line stably express a Gli-responsive Firefly luciferase and a constitutively express a Renilla luciferase as an internal control for cell density, it is widely used to study activation and inhibition of canonical Hedgehog signaling.
The Sonic Hedgehog (Shh) signaling pathway plays a critical role during embryonic development and cancer progression. Various cancers exhibit active Hedgehog signaling. This Gli-mediated transcriptional luciferase reporter assay can be used to evaluate compounds activation/ inhibition of human Gli-mediated transcription.