Product Description

NF-κB Signaling Pathway

NF-κB (Nuclear Factor Kappa B) is a critical transcription factor involved in inflammation, immune responses, cell proliferation, and apoptosis. In its inactive state, NF-κB binds to its inhibitor protein IκB in the cytoplasm. Upon stimulation by external signals (e.g., cytokines, pathogens), IκB is phosphorylated and degraded, releasing NF-κB, which translocates into the nucleus to regulate target gene expression.

AP-1 Signaling Pathway

AP-1 (Activator Protein 1) is a dimeric transcription factor composed of Jun and Fos protein families, playing roles in cell differentiation, proliferation, and apoptosis. Its activation depends on the MAPK pathway (e.g., ERK, JNK, p38). External signals trigger phosphorylation cascades that activate AP-1, enabling it to enter the nucleus and bind specific DNA sequences to regulate gene expression.

Screeningbio’s HEK293/IL-17/Luciferase reporter gene cell line stable transfected with NF-κB and AP-1 response element and luciferase gene. Upon stimulated, NF-κB and AP-1 pathway was activated and induce luciferase expression.