Product Description

The CRE/CREB signaling pathway is a central intracellular mechanism that converts transient extracellular signals into long-term changes in gene expression. When first messengers like neurotransmitters or hormones bind to G-protein coupled receptors, they activate adenylate cyclase, leading to a rise in the second messenger cAMP. This cAMP then activates Protein Kinase A, allowing its catalytic subunits to translocate into the nucleus and phosphorylate the transcription factor CREB at serine residue 133. The phosphorylated CREB binds to the cAMP Response Element in the promoter regions of target genes and recruits coactivators such as CBP/p300. This ultimately initiates the transcription of specific genes involved in critical processes like neuronal plasticity, cell survival, and metabolism.

Screeningbio’s HEK293/CRE/Luciferase reporter gene cell line stable transfected with CREB response element and luciferase gene. Upon stimulated, cAMP/PKA pathway was activated and induce luciferase expression