Product Description

Melanocortin receptor 3 (MC3R) is a G protein-coupled receptor predominantly expressed in the central nervous system, particularly in the hypothalamic arcuate and ventromedial nuclei, and is activated by melanocortins like γ-MSH. Its core function is to act as a “sensor” and “coordinator” of energy homeostasis and feeding behavior. It participates in energy balance by regulating appetite, promoting fat storage, and optimizing energy utilization efficiency, while also coordinating circadian rhythms and reward behaviors. Dysfunction of MC3R is associated with pathological states such as metabolic syndrome and eating disorders.

ScreeningBio’s Rat MC3 β-arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.