Product Description
IRAK4 (Interleukin-1 receptor-associated kinase 4), also known as IPD1, is a member of the serine/threonine protein kinase family (IRAK subfamily) that plays a pivotal role in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways, innate immunity, and inflammatory responses. IRAK4 is activated by various stimuli including IL-1β, IL-18, and pathogen-associated molecular patterns (PAMPs), leading to downstream activation of NF-κB and MAPK pathways via MyD88-dependent signaling, thereby inducing pro-inflammatory cytokine production. Beyond its role in host defense and immune homeostasis, dysregulation of IRAK4 signaling—through loss-of-function mutations or aberrant activation—is closely associated with primary immunodeficiency (e.g., recurrent pyogenic infections), autoinflammatory diseases (e.g., systemic juvenile idiopathic arthritis), and multiple cancers (including diffuse large B-cell lymphoma and colorectal cancer), making it a highly validated target for anti-inflammatory therapy and cancer drug discovery.
Screeningbio’s IRAK4 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying IRAK4 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: IRAK4 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the IRAK4 kinase.
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