Product Description
Insulin-like Growth Factor 1 Receptor (IGF1R), also known as CD221, is a member of the receptor tyrosine kinase (RTK) family that plays a pivotal role in cell growth, proliferation, differentiation, and survival, as well as in embryonic development, tissue homeostasis, and metabolic regulation. IGF1R is activated by ligand-induced autophosphorylation upon binding to its primary ligands, insulin-like growth factor 1 (IGF1) and insulin-like growth factor 2 (IGF2), leading to downstream activation of the PI3K/AKT and RAS/RAF/MAPK signaling pathways, thereby promoting cell cycle progression and inhibiting apoptosis. Beyond its physiological functions in normal growth and metabolism, dysregulation of IGF1R signaling—through overexpression, hyperactivation, or loss of negative regulation—is closely associated with various human cancers (including breast, lung, colorectal, and prostate cancers), as well as growth disorders (e.g., growth hormone insensitivity syndromes) and metabolic diseases such as diabetes and insulin resistance, where it contributes to uncontrolled cell proliferation, therapy resistance, and aberrant metabolic homeostasis, making it a highly validated target for cancer therapy (particularly using tyrosine kinase inhibitors and monoclonal antibodies) and drug discovery.
Screeningbio’s IGF1R Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying IGF1R kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: IGF1R catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the IGF1R kinase.
Data