Product Description
HPK1 (Hematopoietic progenitor kinase 1), also known as MAP4K1 (mitogen-activated protein kinase kinase kinase kinase 1), is a member of the serine/threonine protein kinase family (Ste20-like kinase subfamily) that plays a pivotal role in T-cell receptor (TCR) signaling, B-cell receptor signaling, and immune homeostasis, as well as in apoptosis, cell migration, and antigen receptor-induced negative regulation of T-cell activation. HPK1 is activated by phosphorylation through various stimuli including TCR engagement, oxidative stress, and growth factor deprivation, leading to downstream activation of JNK, p38 MAPK, and NF-κB pathways, as well as negative regulation of proximal TCR signaling by phosphorylating SLP-76. Beyond its role in normal immune cell development and tolerance, dysregulation of HPK1 signaling—through reduced expression, loss-of-function mutations, or impaired kinase activity—is closely associated with autoimmunity, chronic inflammation, and multiple cancers (including breast cancer, pancreatic cancer, and leukemia), where it functions as a key negative regulator of antitumor immunity, making it a highly validated target for cancer immunotherapy (particularly for checkpoint inhibition) and drug discovery.
Screeningbio’s HPK1 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying HPK1 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: HPK1 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the HPK1 kinase.
Data