Product Description
HER4 (Human epidermal growth factor receptor 4), also known as ERBB4 (avian erythroblastic leukemia viral oncogene homolog 4), is a member of the receptor tyrosine kinase (RTK) family (subclass I, EGFR family) that plays a pivotal role in cell proliferation, differentiation, survival, and development of the nervous system, heart, and mammary gland. HER4 binds with high affinity to multiple ligands including neuregulins (NRG1, NRG2, NRG3, NRG4), epiregulin, betacellulin, and heparin-binding EGF (HB-EGF), leading to activation of downstream signaling pathways such as RAS-MAPK, PI3K-AKT, and PLCγ. Beyond its role in normal cardiac and neuronal development, tissue repair, and endocrine differentiation, dysregulation of HER4 signaling—through mutations (e.g., in the kinase domain), alternative splicing variants (e.g., JM-a, JM-b, CYT-1, CYT-2), or altered expression—is closely associated with breast cancer, colorectal cancer, lung cancer, glioblastoma, and heart failure, playing context-dependent tumor-suppressive or oncogenic roles, making it a potential target for cancer therapy and drug discovery.
Screeningbio’s HER4 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying HER4 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: HER4 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the HER4 kinase.
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