Product Description

FLT1 (Fms-like tyrosine kinase 1), also known as VEGFR1 (Vascular Endothelial Growth Factor Receptor 1), is a member of the receptor tyrosine kinase (RTK) family that plays a pivotal role in angiogenesis, vasculogenesis, and pathological cell proliferation. FLT1 binds with high affinity to ligands such as VEGF-A, VEGF-B, and Placental Growth Factor (PlGF). Beyond its role in vascular development, dysregulation of FLT1 signaling is closely associated with tumor angiogenesis, inflammatory diseases, and metastasis, making it a highly validated target for cancer therapy and drug discovery.

Screeningbio’s FLT1 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying FLT1 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).

This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.

The assay is performed in two distinct steps:

1. Kinase Reaction: FLT1 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.

2. ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.

3. Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.

The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the FLT1 kinase.