Product Description
DDR2 (Discoidin Domain Receptor 2) is a member of the receptor tyrosine kinase (RTK) family that plays a pivotal role in cell proliferation, migration, matrix remodeling, and tissue morphogenesis. DDR2 binds with high affinity to various types of collagen (primarily type I and type III) as its activating ligands, distinguishing it from other RTKs that are typically activated by soluble growth factors. Collagen binding induces sustained DDR2 autophosphorylation and activates downstream signaling pathways, including MAPK, PI3K/AKT, and Src family kinases. Beyond its essential physiological functions in skeletal development, wound healing, and connective tissue homeostasis, dysregulation of DDR2 signaling—including gain-of-function mutations, overexpression, and aberrant collagen-dependent activation—is closely associated with tumor progression (such as lung squamous cell carcinoma, breast cancer, and esophageal cancer), fibrosis (including pulmonary and liver fibrosis), and skeletal disorders. These features have established DDR2 as a highly validated target for cancer therapy and drug discovery.
Screeningbio’s DDR2 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying DDR2 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: DDR2 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the DDR2 kinase.
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