Product Description
CK1α1 (Casein kinase 1 alpha 1), also known as CSNK1A1, is a member of the serine/threonine kinase family that plays a pivotal role in regulating diverse cellular processes, including Wnt/β-catenin signaling, cell cycle progression, circadian rhythm, and membrane trafficking. CK1α1 phosphorylates key substrates such as β-catenin, p53, and components of the Wnt pathway, thereby controlling protein stability, localization, and transcriptional activity. Beyond its essential physiological functions in development and cellular homeostasis, dysregulation of CK1α1 signaling—including loss-of-function mutations, altered expression, and aberrant pathway modulation—is closely associated with various cancers (such as chronic lymphocytic leukemia, colorectal cancer, and breast cancer), neurodegenerative diseases, and disorders of circadian rhythm, making it a highly validated target for cancer therapy and drug discovery.
Screeningbio’s CK1α1 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying CK1α1 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: CK1α1 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the CK1α1 kinase.
Data