Product Description
CAMKK1 (Calcium/calmodulin-dependent protein kinase kinase 1), also known as CaMKKα, is a member of the serine/threonine kinase family that plays a pivotal role in calcium signaling by phosphorylating and activating downstream kinases such as CAMK1, CAMK4, and AMPK. CAMKK1 is activated by increases in intracellular calcium levels and calmodulin binding, serving as a key upstream regulator of multiple signaling pathways involved in energy homeostasis, neuronal development, and cell survival. Beyond its essential physiological functions, dysregulation of CAMKK1 signaling is closely associated with metabolic disorders (including obesity and type 2 diabetes), neuropsychiatric diseases, and cancer progression, making it a highly validated target for drug discovery and therapeutic intervention.
Screeningbio’s CAMKK1 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying CAMKK1 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: CAMKK1 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the CAMKK1 kinase.
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