Product Description
CAMK4 (Calcium/calmodulin-dependent protein kinase type IV), also known as CaMKIV, is a member of the serine/threonine kinase family that plays a pivotal role in calcium-regulated gene expression, immune responses, and neuronal synaptic plasticity. CAMK4 is activated by calcium-bound calmodulin and subsequent phosphorylation by upstream kinases such as CaMKK, leading to the phosphorylation of transcription factors including CREB, MEF2, and HDACs. Beyond its essential physiological functions in long-term memory formation, T-cell activation, and neuronal survival, dysregulation of CAMK4 signaling—including aberrant expression and altered activity—is closely associated with neurodevelopmental disorders, autoimmune diseases, inflammatory conditions, and cancer progression, making it a highly validated target for therapeutic intervention in neurology, immunology, and oncology.
Screeningbio’s CAMK4 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying CAMK4 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: CAMK4 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo ™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the CAMK4 kinase.
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