Product Description
ASK1(Apoptosis Signal-regulating Kinase 1), also known as MAP3K5, is a member of the serine/threonine kinase family and a key component of the MAP kinase signaling cascade that plays a pivotal role in cellular stress responses, inflammation, and apoptosis. ASK1 is activated by various stress stimuli including reactive oxygen species (ROS), TNF-α, and endoplasmic reticulum stress, leading to downstream activation of the JNK and p38 MAPK pathways. Beyond its essential physiological functions in stress-induced apoptosis and immune regulation, dysregulation of ASK1 signaling is closely associated with neurodegenerative diseases (such as Alzheimer's and Parkinson's disease), cardiovascular disorders, inflammatory diseases, and cancer, making it a highly validated target for therapeutic intervention in multiple disease areas.
Screeningbio’s ASK1 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying ASK1 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: ASK1 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the ASK1 kinase.
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