Product Description
ALK2 (Activin Receptor-Like Kinase 2), also known as ACVR1 (Activin A Receptor Type 1), is a member of the serine/threonine kinase family and functions as a type I receptor for bone morphogenetic proteins (BMPs), playing a pivotal role in embryonic development, bone formation, and tissue homeostasis. ALK2 transduces signals upon ligand binding by forming complexes with type II receptors and subsequently phosphorylating downstream SMAD transcription factors. Beyond its physiological functions in skeletal development and iron metabolism, dysregulation of ALK2—particularly through activating mutations such as the classic R206H substitution—is the primary driver of fibrodysplasia ossificans progressiva (FOP), a rare disorder characterized by heterotopic ossification. Additionally, aberrant ALK2 signaling has been implicated in diffuse intrinsic pontine glioma (DIPG) and other cancers, making it a highly validated target for therapeutic intervention in both rare genetic diseases and oncology.
Screeningbio’s ALK2 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying ALK2 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: ALK2 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the ALK2 kinase.
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