Product Description

The STAT5 signaling pathway is a core member of the JAK-STAT family, primarily activated by cytokines such as interleukins and growth hormone. Upon ligand binding to its membrane receptor, the associated JAK kinases cross-phosphorylate and activate each other. They then phosphorylate specific tyrosine residues on the receptor's intracellular domain and on STAT5 proteins. The phosphorylated STAT5 forms dimers, translocates into the nucleus, and functions as a transcription factor by directly binding to DNA to regulate target gene expression. This process governs critical cellular responses including proliferation, differentiation, survival, and immune function, playing a particularly vital role in hematopoiesis and immune regulation. Its constitutive activation is closely associated with the development of various leukemias and solid tumors.

Screeningbio’s U937/STAT5/Luciferase reporter gene cell line stable transfected with STAT-5 response element and luciferase gene. Upon stimulated, STAT-5 pathway was activated and induce luciferase expression.