Jurkat NF-κB Luciferase Reporter Cell Line

Jurkat NF-κB-Luciferase Reporter Cell Line

Item	Cat#	Price
Stable Cell Line	SNB-SP-0007	$1,980
Compound Test Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

The NF-κB (Nuclear Factor Kappa B) signaling pathway is a central pathway regulating inflammation, immunity, cell survival, and proliferation. In its inactive state, NF-κB (typically a p50/p65 dimer) is bound to the inhibitory protein IκB and sequestered in the cytoplasm. Upon cellular stimulation by external signals (e.g., TNF-α, bacterial LPS), the IκB kinase (IKK) complex is activated, leading to phosphorylation and subsequent ubiquitin-mediated degradation of IκB. The released NF-κB then translocates into the nucleus, where it binds to specific DNA sequences to initiate the transcription of various target genes (such as cytokines, chemokines, and anti-apoptotic proteins), thereby participating in immune responses, inflammatory reactions, and tumorigenesis.

Screeningbio’s Jurkat/NF-κB/Luciferase reporter gene cell line stable transfected with NF-κB response element and luciferase gene. Upon stimulated, NF-κB pathway was activated and induce luciferase expression.

Product Specifications

Target Type	Signal Pathway
Species	Human
HGNC Symbol
Accession Number
Parental Line	Jurkat
Lot#	See Vial
Storage	Liquid Nitrogen

Data

Jurkat/NF-κB/Luciferase Agonist Assay. Jurkat/NF-κB/Luciferase reporter cells were treated with TNFα. The assay was run based on Promega ONE-GLO Luciferase Assay System. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software. — **Jurkat/NF-κB/Luciferase Agonist Assay.** Jurkat/NF-κB/Luciferase reporter cells were treated with TNFα. The assay was run based on Promega ONE-GLOLuciferase Assay System. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software.

Target Background

The NF-κB (Nuclear Factor Kappa B) signaling pathway is a central pathway regulating inflammation, immunity, cell survival, and proliferation. In its inactive state, NF-κB (typically a p50/p65 dimer) is bound to the inhibitory protein IκB and sequestered in the cytoplasm. Upon cellular stimulation by external signals (e.g., TNF-α, bacterial LPS), the IκB kinase (IKK) complex is activated, leading to phosphorylation and subsequent ubiquitin-mediated degradation of IκB.

The released NF-κB then translocates into the nucleus, where it binds to specific DNA sequences to initiate the transcription of various target genes (such as cytokines, chemokines, and anti-apoptotic proteins), thereby participating in immune responses, inflammatory reactions, and tumorigenesis.