Product Description

The NF-κB (Nuclear Factor Kappa B) signaling pathway is a central pathway regulating inflammation, immunity, cell survival, and proliferation. In its inactive state, NF-κB (typically a p50/p65 dimer) is bound to the inhibitory protein IκB and sequestered in the cytoplasm. Upon cellular stimulation by external signals (e.g., TNF-α, bacterial LPS), the IκB kinase (IKK) complex is activated, leading to phosphorylation and subsequent ubiquitin-mediated degradation of IκB. The released NF-κB then translocates into the nucleus, where it binds to specific DNA sequences to initiate the transcription of various target genes (such as cytokines, chemokines, and anti-apoptotic proteins), thereby participating in immune responses, inflammatory reactions, and tumorigenesis. 

Screeningbio’s Jurkat/NF-κB/Luciferase reporter gene cell line stable transfected with NF-κB response element and luciferase gene. Upon stimulated, NF-κB pathway was activated and induce luciferase expression.