Product Description

The SRE (Serum Response Element) signaling pathway is a key mechanism regulating cell growth, proliferation, and differentiation, primarily responding to signals such as growth factors (e.g., EGF), serum stimulation, and cellular stress. SRE is a specific DNA sequence located in the promoter regions of target genes, recognized and bound by the transcription factor SRF (Serum Response Factor). Upon activation of the Ras-MAPK pathway by external signals, downstream kinases (e.g., RSK) phosphorylate SRF or its coactivators (e.g., Ternary Complex Factors, TCFs), facilitating stable binding of the SRF complex to SRE sites. This initiates the transcription of immediate early genes such as c-fos, thereby further regulating cell cycle progression and differentiation.

Screeningbio’s HEK293/SRE/Luciferase reporter gene cell line stable transfected with SRE response element and luciferase gene. Upon stimulated, SRE pathway was activated and induce luciferase expression.