HEK293 Human TRPV4 Stable Cell
Item | Cat# | Price |
Stable Cell Line | SNB-I-0009A | $19,800 |
Compound Test Services | CT-001 | $1,850 per 384w plate (Up To 16 cpds Dose) |
Product Description
Transient Receptor Potential Vanilloid subtype 4 (TRPV4) channels are a diverse family of non-selective cation channels involved in sensing a wide range of physical and chemical stimuli, including temperature, mechanical forces, pH, and ligands such as capsaicin and menthol. They are broadly expressed in sensory neurons and other tissues, playing essential roles in pain perception, thermoregulation, inflammation, and cellular homeostasis. The TRP channel family is divided into several subfamilies, including TRPC, TRPV, TRPM, TRPA, TRPP, and TRPML, each with distinct physiological functions and activation mechanisms. Due to their central role in sensory signaling and disease, TRP channels are emerging as promising therapeutic targets for pain, inflammation, and various neurological and cardiovascular disorders.
Screeningbio’s TRPV4 cell line stable express non-tag full length human TRPV4 receptor in HEK293 cell. When activated, TRPV4 cell line response to extracellular stimuli and result in channel opening and calcium influx. Increase of intercellular calcium was detected by calcium sensitive dye.
Product Specifications
Target Type | Ion Channel |
Species | Human |
HGNC Symbol | TRPV4 |
Accession Number | NM_021625 |
Parental Line | HEK293 |
Lot# | See Vial |
Storage | Liquid Nitrogen |
Data
![Human TRPV4 Blocker Assay. HEK293 Human TRPV4 cells were seeded in 384-well plate and incubated at 37oC in 5% CO2 incubator for 24 hours before running the assay. The cells were treated with the reference blockers, and stimulated by activator. The assay was run based on FLIPR Calcium assay protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software.](https://static.wixstatic.com/media/56275b_206b17ae277c43ec930275de69f18863~mv2.png/v1/fill/w_76,h_75,al_c,q_85,usm_0.66_1.00_0.01,blur_2,enc_auto/56275b_206b17ae277c43ec930275de69f18863~mv2.png)
Target Background
The transient receptor potential vanilloid 4 (TRPV4) is a non-selective cation channel within the TRP superfamily that functions as a polymodal sensor responsive to mechanical, thermal, and osmotic stimuli. TRPV4 is broadly expressed in many tissues, including sensory neurons, endothelial cells, epithelial cells, chondrocytes, and the urinary bladder. It is activated by moderate warmth (approximately 27–35 °C), cell swelling, shear stress, and endogenous lipid mediators such as epoxyeicosatrienoic acids (EETs). Through these mechanisms, TRPV4 serves as an essential transducer of mechanical and osmotic signals, linking environmental and physiological cues to cellular responses.
Upon activation, TRPV4 mediates calcium and sodium influx, triggering downstream pathways that regulate cell volume, cytoskeletal organization, and inflammatory signaling. In sensory neurons, TRPV4 contributes to mechanosensation, osmotic detection, and pain transduction. In non-neuronal tissues, it plays critical roles in vascular tone regulation, epithelial barrier function, pulmonary fluid balance, and skeletal development.
Aberrant TRPV4 function has been implicated in various diseases, including neuropathic pain, pulmonary edema, osteoarthritis, skeletal dysplasias, and hereditary motor neuropathies. Because of its involvement in both mechanical and inflammatory processes, TRPV4 has emerged as a promising therapeutic target for conditions involving edema, joint degeneration, and chronic pain. Its diverse physiological roles continue to make TRPV4 an active area of investigation in sensory biology, cardiovascular regulation, and tissue homeostasis.