HEK293 Human TRPV3 Stable Cell
Item | Cat# | Price |
Stable Cell Line | SNB-I-0008A | $19,800 |
Compound Test Services | CT-001 | $1,850 per 384w plate (Up To 16 cpds Dose) |
Product Description
Transient Receptor Potential Vanilloid subtype 3 (TRPV3) channels are a diverse family of non-selective cation channels involved in sensing a wide range of physical and chemical stimuli, including temperature, mechanical forces, pH, and ligands such as capsaicin and menthol. They are broadly expressed in sensory neurons and other tissues, playing essential roles in pain perception, thermoregulation, inflammation, and cellular homeostasis. The TRP channel family is divided into several subfamilies, including TRPC, TRPV, TRPM, TRPA, TRPP, and TRPML, each with distinct physiological functions and activation mechanisms. Due to their central role in sensory signaling and disease, TRP channels are emerging as promising therapeutic targets for pain, inflammation, and various neurological and cardiovascular disorders.
Screeningbio’s TRPV3 cell line stable express non-tag full length human TRPV3 receptor in HEK293 cell. When activated, TRPV3 cell line response to extracellular stimuli and result in channel opening and calcium influx. Increase of intercellular calcium was detected by calcium sensitive dye.
Product Specifications
Target Type | Ion Channel |
Species | Human |
HGNC Symbol | TRPV3 |
Accession Number | NM_145068 |
Parental Line | HEK293 |
Lot# | See Vial |
Storage | Liquid Nitrogen |
Data
![Human TRPV3 Blocker Assay. HEK293 Human TRPV3 cells were seeded in 384-well plate and incubated at 37oC in 5% CO2 incubator for 24 hours before running the assay. The cells were treated with the reference blockers, and stimulated by activator. The assay was run based on FLIPR Calcium assay protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software.](https://static.wixstatic.com/media/56275b_811957a1913b4ccaad25c8d5a6680f7f~mv2.png/v1/fill/w_76,h_75,al_c,q_85,usm_0.66_1.00_0.01,blur_2,enc_auto/56275b_811957a1913b4ccaad25c8d5a6680f7f~mv2.png)
Target Background
The transient receptor potential vanilloid 3 (TRPV3) is a non-selective cation channel belonging to the TRP superfamily and is closely related to TRPV1 and TRPV2 in both structure and function. TRPV3 is primarily expressed in epithelial tissues such as skin keratinocytes, as well as in sensory neurons, the brain, and other organs including the testis and gastrointestinal tract. Unlike TRPV1, TRPV3 is activated by moderate heat in the range of 31–39 °C, as well as by various natural and synthetic compounds such as camphor, eugenol, and 2-APB, enabling it to act as a molecular sensor for warm temperatures and chemical stimuli.
Upon activation, TRPV3 allows the influx of calcium and sodium ions, leading to membrane depolarization and activation of downstream signaling cascades involved in thermosensation, skin barrier formation, and inflammatory responses. In keratinocytes, TRPV3-mediated calcium signaling regulates cell differentiation, hair growth, and the release of paracrine mediators that influence sensory neurons, thereby linking skin physiology to sensory perception.
Dysregulation or mutation of TRPV3 has been associated with several pathological conditions, including Olmsted syndrome (a severe skin hyperkeratosis disorder), itch, pain hypersensitivity, and dermatitis. Pharmacological modulation of TRPV3 offers potential therapeutic strategies for treating chronic itch, pain, and skin barrier dysfunction. Given its unique localization and role in skin homeostasis, TRPV3 continues to be an important focus of research in sensory biology and dermatological therapeutics.