HEK293 Human TRPV2 Stable Cell
Item | Cat# | Price |
Stable Cell Line | SNB-I-0007A | $19,800 |
Compound Test Services | CT-001 | $1,850 per 384w plate (Up To 16 cpds Dose) |
Product Description
Transient Receptor Potential Vanilloid subtype 2 (TRPV2) channels are a diverse family of non-selective cation channels involved in sensing a wide range of physical and chemical stimuli, including temperature, mechanical forces, pH, and ligands such as capsaicin and menthol. They are broadly expressed in sensory neurons and other tissues, playing essential roles in pain perception, thermoregulation, inflammation, and cellular homeostasis. The TRP channel family is divided into several subfamilies, including TRPC, TRPV, TRPM, TRPA, TRPP, and TRPML, each with distinct physiological functions and activation mechanisms. Due to their central role in sensory signaling and disease, TRP channels are emerging as promising therapeutic targets for pain, inflammation, and various neurological and cardiovascular disorders.
Screeningbio’s TRPV2 cell line stable express non-tag full length human TRPV2 receptor in HEK293 cell. When activated, TRPV2 cell line response to extracellular stimuli and result in channel opening and calcium influx. Increase of intercellular calcium was detected by calcium sensitive dye.
Product Specifications
Target Type | Ion Channel |
Species | Human |
HGNC Symbol | TRPV2 |
Accession Number | NM_016113 |
Parental Line | HEK293 |
Lot# | See Vial |
Storage | Liquid Nitrogen |
Data
![Human TRPV2 Blocker Assay. HEK293 Human TRPV2 cells were seeded in 384-well plate and incubated at 37oC in 5% CO2 incubator for 24 hours before running the assay. The cells were treated with the reference blockers, and stimulated by activator. The assay was run based on FLIPR Calcium assay protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software.](https://static.wixstatic.com/media/56275b_4155c8aecde44d58bba385eb9de59bf7~mv2.png/v1/fill/w_76,h_75,al_c,q_85,usm_0.66_1.00_0.01,blur_2,enc_auto/56275b_4155c8aecde44d58bba385eb9de59bf7~mv2.png)
Target Background
The transient receptor potential vanilloid 2 (TRPV2) is a non-selective cation channel that belongs to the TRP channel superfamily and shares structural and functional homology with TRPV1. Unlike TRPV1, TRPV2 is activated by higher temperature thresholds (>52 °C) and is insensitive to capsaicin or protons, distinguishing it as a high-threshold heat sensor. TRPV2 is widely expressed in various tissues, including sensory neurons, cardiac and skeletal muscle, immune cells, and epithelial tissues, suggesting diverse physiological roles beyond thermosensation.
Upon activation, TRPV2 mediates calcium and sodium influx, contributing to membrane depolarization and the regulation of cellular processes such as growth, migration, and survival. In immune cells like macrophages and mast cells, TRPV2 modulates phagocytosis, cytokine release, and inflammatory signaling. Its activity is regulated by membrane trafficking, phosphorylation, and interactions with signaling molecules such as PI3K, allowing dynamic responses to mechanical stretch, growth factors, and osmotic stress.
Functionally, TRPV2 plays critical roles in mechanosensation, cardiac contractility, insulin secretion, and immune responses. Dysregulation of TRPV2 has been implicated in pathological conditions including muscular dystrophy, cardiomyopathy, cancer progression, and chronic inflammation. Although its precise gating mechanisms and physiological modulators remain under active investigation, TRPV2 represents an emerging therapeutic target for disorders involving inflammation, metabolism, and cancer cell invasiveness.