Product Description

The cannabinoid receptor 2 (CB2) is a G protein-coupled receptor within the endocannabinoid system, primarily distributed in the peripheral immune system (e.g., spleen, tonsils, and immune cells like B cells, T cells, and macrophages), and also expressed in microglia of the central nervous system. Its core function is to modulate immune responses and inflammation; activation suppresses pro-inflammatory cytokine release, exerting anti-inflammatory and immunomodulatory effects. Thus, CB2 is a non-psychoactive potential target for treating inflammatory diseases, neuropathic pain, and autoimmune disorders.

ScreeningBio’s HEK293T/Mouse CB2 β-arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.