HEK293T/Human D2S β-Arrestin Stable Cell
Item | Cat# | Price |
Stable Cell Line | SNB-A-0083E | Inquiry |
Compound Testing Services | CT-001 | $1,850 per 384w plate (Up To 16 cpds Dose) |
Product Description
The dopamine D2 receptor short isoform is a major splice variant of the D2 receptor characterized by a shorter third intracellular loop. It is predominantly localized to the presynaptic terminals of midbrain dopaminergic neurons, where it functions as a critical autoreceptor. At the cellular level, it provides negative feedback to inhibit dopamine synthesis and release via Gi/o signaling pathways, thereby finely tuning the strength and duration of dopaminergic transmission. It is also found on some postsynaptic membranes. Due to its unique functions, D2S is a key molecule in regulating motor function, reward mechanisms, and the pathophysiology of schizophrenia, making it one of the important targets for antipsychotic drugs.
ScreeningBio’s HEK293T/Human D2S β-Arrestin cell line co-express the SmBiT tagged Human D2S receptor and the LgBiT tagged β-Arrestin. Activation of the GPCR-SmBiT induces β-Arrestin-LgBiT recruitment, forcing complementation of two NanoBiT enzyme fragments (SmBiT and LgBiT). The resulting functional NanoBiT enzyme catalyze substrate to generate a luminescent signal. Promega Nano-Glo Live Cell Kit can be used to detect the signal.
Product Specifications
Target Type | GPCR |
Species | Human |
HGNC Symbol | DRD2S |
Accession Number | NM_016574 (Hs) |
Parental Line | HEK293T |
Lot# | See Vial |
Storage | Liquid Nitrogen |
Data
Target Background
The dopamine D2 receptor short isoform is a major splice variant of the D2 receptor characterized by a shorter third intracellular loop. It is predominantly localized to the presynaptic terminals of midbrain dopaminergic neurons, where it functions as a critical autoreceptor. At the cellular level, it provides negative feedback to inhibit dopamine synthesis and release via Gi/o signaling pathways, thereby finely tuning the strength and duration of dopaminergic transmission.
It is also found on some postsynaptic membranes. Due to its unique functions, D2S is a key molecule in regulating motor function, reward mechanisms, and the pathophysiology of schizophrenia, making it one of the important targets for antipsychotic drugs.