HEK293 Pig MRGPRX2 Stable Cell

HEK293/Pig MRGPRX2 Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0270N	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

MRGPRX2 (Mas-related G protein-coupled receptor member X2) is a G protein-coupled receptor (GPCR) primarily expressed in mast cells and certain neural tissues. It is involved in various physiological and pathological processes, including pain sensation, immune responses, and inflammation. The neuro-immune hyperactivation mediated by MRGPRX2 is a common feature of many chronic diseases, such as allergy, autoimmunity, pain, and pruritus.

Screeningbio’s HEK293/Pig MRGPRX2 cell line overexpress MRGPRX2 receptor and is designed to detect increases in intracellular Calcium flux signal in response to agonist stimulation of the receptor. Molecular Devices Calcium 6 kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Pig
HGNC Symbol	MRGPRX2
Accession Number	XM_013994496.2
Parental Line	HEK293
Lot#	See Vial
Storage	Liquid Nitrogen

Data

HEK293/Pig MRGPRX2 Agonist Assay. HEK293/Pig MRGPRX2 cells were treated with the reference agonist Substance-P. The assay was run based on FLIPR Calcium 6 Assay protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software. — **HEK293/Pig MRGPRX2 Agonist Assay.** HEK293/Pig MRGPRX2 cells were treated with the reference agonist Substance-P. The assay was run based on FLIPR Calcium 6 Assay protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software.

Target Background

MRGPRX2 (Mas-related G protein-coupled receptor member X2) is a G protein-coupled receptor (GPCR) primarily expressed in mast cells and certain neural tissues. It is involved in various physiological and pathological processes, including pain sensation, immune responses, and inflammation. The neuro-immune hyperactivation mediated by MRGPRX2 is a common feature of many chronic diseases, such as allergy, autoimmunity, pain, and pruritus.