HEK293 Human PTH1 Stable Cell

HEK293/Human PTH1 Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0188A	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

Parathyroid hormone receptor 1 (PTH1R) is the primary class B G protein-coupled receptor mediating the actions of parathyroid hormone, mainly distributed in the kidneys and bones (osteoblasts and osteocytes), serving as the central target for calcium and phosphate metabolism. Its core function, through activation of multiple pathways including Gs/cAMP and Gq/PKC, is to promote calcium reabsorption and inhibit phosphate reabsorption in the kidneys, while dually regulating bone resorption and formation (depending on the mode of action) in bone, thereby jointly maintaining blood calcium homeostasis and bone metabolic balance. Abnormalities in this receptor are directly linked to various bone metabolic disorders (e.g., osteoporosis, hyperparathyroidism) and genetic diseases (e.g., Jansen's metaphyseal chondrodysplasia).

Screeningbio’s HEK293/Human PTH1 cell line overexpress PTH1R and is designed to detect increases in intracellular cAMP levels in response to agonist stimulation of the receptor. Cisbio HTRF cAMP kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Human
HGNC Symbol	PTH1R
Accession Number	SNB-G-0188A
Parental Line	HEK293
Lot#	See Vial
Storage	Liquid Nitrogen

Data

HEK293/Human PTH1 Agonist Assay. HEK293/Human PTH1 cells were treated with the reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software. — **HEK293/Human PTH1 Agonist Assay.** HEK293/Human PTH1 cells were treated with the reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software.

Target Background

Parathyroid hormone receptor 1 (PTH1R) is the primary class B G protein-coupled receptor mediating the actions of parathyroid hormone, mainly distributed in the kidneys and bones (osteoblasts and osteocytes), serving as the central target for calcium and phosphate metabolism.

Its core function, through activation of multiple pathways including Gs/cAMP and Gq/PKC, is to promote calcium reabsorption and inhibit phosphate reabsorption in the kidneys, while dually regulating bone resorption and formation (depending on the mode of action) in bone, thereby jointly maintaining blood calcium homeostasis and bone metabolic balance.

Abnormalities in this receptor are directly linked to various bone metabolic disorders (e.g., osteoporosis, hyperparathyroidism) and genetic diseases (e.g., Jansen's metaphyseal chondrodysplasia).