HEK293 Human ETB Stable Cell

HEK293/Human ETB Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0088A	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

ETB (EDNRB), the endothelin type-B receptor, is a G protein-coupled receptor belonging to the same family as ETA. It is widely distributed in vascular endothelial cells, the kidneys (e.g., renal tubules and collecting ducts), the central nervous system, the intestines, and some smooth muscle tissues. Its core function involves dual regulatory roles: on vascular endothelium, ETB receptor activation promotes the release of nitric oxide and prostacyclin, leading to vasodilation, and it helps clear circulating endothelin-1 to regulate its levels; in the kidneys, it participates in regulating water-salt balance and blood pressure; concurrently, it also mediates cell proliferation, migration, and nervous system development and function.

Screeningbio’s HEK293/Human ETB cell line overexpress EDNRB and is designed to detect increases in intracellular IP-1 levels in response to agonist stimulation of the receptor. Cisbio HTRF IP-1 kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Human
HGNC Symbol	EDNRB
Accession Number	NM_000115
Parental Line	HEK293
Lot#	See Vial
Storage	Liquid Nitrogen

Data

HEK293/Human ETB Agonist Assay. HEK293/Human ETB cells were treated with the reference agonist. The assay was run based on Revvity IP-one HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software. — **HEK293/Human ETB Agonist Assay.** HEK293/Human ETB cells were treated with the reference agonist. The assay was run based on Revvity IP-one HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software.

Target Background

ETB (EDNRB), the endothelin type-B receptor, is a G protein-coupled receptor belonging to the same family as ETA. It is widely distributed in vascular endothelial cells, the kidneys (e.g., renal tubules and collecting ducts), the central nervous system, the intestines, and some smooth muscle tissues.

Its core function involves dual regulatory roles: on vascular endothelium, ETB receptor activation promotes the release of nitric oxide and prostacyclin, leading to vasodilation, and it helps clear circulating endothelin-1 to regulate its levels; in the kidneys, it participates in regulating water-salt balance and blood pressure; concurrently, it also mediates cell proliferation, migration, and nervous system development and function.