HEK293 Human D1 Stable Cell

HEK293/Human D1 Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0082A	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

Dopamine receptor D1 (D1) is a primary Gs protein-coupled receptor in the dopamine receptor family, mediating the excitatory effects of dopamine. It is highly concentrated in the striatum, nucleus accumbens, and cerebral cortex of the brain, and is also found in the kidneys and vascular smooth muscle. Its key functions include enhancing neuronal excitability, regulating motor control, reinforcing reward and motivational behaviors, promoting memory and cognitive functions, and modulating renal sodium excretion and vasodilation. Consequently, the D1 receptor is a significant therapeutic target for treating Parkinson's disease, cognitive impairment, negative symptoms of schizophrenia, and for blood pressure regulation.

Screeningbio’s HEK293/Human D1 cell line overexpress DRD1 and is designed to detect increases in intracellular cAMP levels in response to agonist stimulation of the receptor. Cisbio HTRF cAMP kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Human
HGNC Symbol	DRD1
Accession Number	NM_000794
Parental Line	HEK293
Lot#	See Vial
Storage	Liquid Nitrogen

Data

HEK293/Human D1 Agonist Assay. HEK293/Human D1 cells were treated with the reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software. — **HEK293/Human D1 Agonist Assay.** HEK293/Human D1 cells were treated with the reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software.

Target Background

Dopamine receptor D1 (D1) is a primary Gs protein-coupled receptor in the dopamine receptor family, mediating the excitatory effects of dopamine. It is highly concentrated in the striatum, nucleus accumbens, and cerebral cortex of the brain, and is also found in the kidneys and vascular smooth muscle.

Its key functions include enhancing neuronal excitability, regulating motor control, reinforcing reward and motivational behaviors, promoting memory and cognitive functions, and modulating renal sodium excretion and vasodilation.

Consequently, the D1 receptor is a significant therapeutic target for treating Parkinson's disease, cognitive impairment, negative symptoms of schizophrenia, and for blood pressure regulation.