HEK293 Canis CCK2 Stable Cell

HEK293/Canis CCK2 Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0076M	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

The Cholecystokinin B receptor (CCK2 or CCK-B) is a G protein-coupled receptor with high affinity for both cholecystokinin and gastrin. It is widely expressed in the central nervous system (e.g., cerebral cortex, limbic system) and the gastrointestinal tract (e.g., gastric parietal cells, certain intestinal cells). Its primary functions involve mediating neuromodulatory effects in the CNS, regulating anxiety, pain perception, memory, and dopaminergic neuron activity. Peripherally, it potently stimulates gastric acid secretion from parietal cells and may influence cell proliferation. Consequently, the CCK2 receptor is a significant therapeutic target for anxiety disorders, analgesia, and gastric acid-related conditions such as gastrinoma.

Screeningbio’s HEK293/Canis CCK2 cell line overexpress CCK2 receptor and is designed to detect increases in intracellular IP-1 levels in response to agonist stimulation of the receptor. Cisbio HTRF IP-1 kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Canis
HGNC Symbol	CCKBR
Accession Number	XM_038430347
Parental Line	HEK293
Lot#	See Vial
Storage	Liquid Nitrogen

Data

HEK293/Canis CCK2 Agonist Assay. HEK293/Canis CCK2 cells were treated with the reference agonist. The assay was run based on Revvity IP-one HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software. — **HEK293/Canis CCK2 Agonist Assay.** HEK293/Canis CCK2 cells were treated with the reference agonist. The assay was run based on Revvity IP-one HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software.

Target Background

The Cholecystokinin B receptor (CCK2 or CCK-B) is a G protein-coupled receptor with high affinity for both cholecystokinin and gastrin. It is widely expressed in the central nervous system (e.g., cerebral cortex, limbic system) and the gastrointestinal tract (e.g., gastric parietal cells, certain intestinal cells). Its primary functions involve mediating neuromodulatory effects in the CNS, regulating anxiety, pain perception, memory, and dopaminergic neuron activity.

Peripherally, it potently stimulates gastric acid secretion from parietal cells and may influence cell proliferation. Consequently, the CCK2 receptor is a significant therapeutic target for anxiety disorders, analgesia, and gastric acid-related conditions such as gastrinoma.