CHO-K1/Rat H2 Stable Cell
Item | Cat# | Price |
Stable Cell Line | SNB-G-0116C | $19,800 |
Compound Testing Services | CT-001 | $1,850 per 384w plate (Up To 16 cpds Dose) |
Product Description
The histamine H2 receptor (HRH2) is a G protein-coupled receptor primarily mediating gastric acid secretion. It is predominantly located in gastric mucosal parietal cells, with additional expression in cardiomyocytes, vascular smooth muscle, immune cells, and the central nervous system. Its function is achieved by activating the Gs protein-adenylyl cyclase-cAMP signaling pathway upon histamine binding, potently stimulating gastric acid secretion from parietal cells and partially promoting pepsinogen release. Furthermore, it contributes to positive chronotropic effects in the heart and modulates cytokine secretion in immune cells. This receptor is a classic therapeutic target for peptic ulcers and gastroesophageal reflux disease, with H2 receptor antagonists (e.g., ranitidine, famotidine) effectively inhibiting excessive acid secretion by blocking this pathway.
Screeningbio’s CHO-K1/Rat H2 cell line overexpress HRH2 and is designed to detect increases in intracellular cAMP levels in response to agonist stimulation of the receptor. Cisbio HTRF cAMP kit can be used to detect the signal.
Product Specifications
Target Type | GPCR |
Species | Rat |
HGNC Symbol | HRH2 |
Accession Number | NM_012965 (Rn) |
Parental Line | CHO-K1 |
Lot# | See Vial |
Storage | Liquid Nitrogen |
Data
Target Background
The histamine H2 receptor (HRH2) is a G protein-coupled receptor primarily mediating gastric acid secretion. It is predominantly located in gastric mucosal parietal cells, with additional expression in cardiomyocytes, vascular smooth muscle, immune cells, and the central nervous system.
Its function is achieved by activating the Gs protein-adenylyl cyclase-cAMP signaling pathway upon histamine binding, potently stimulating gastric acid secretion from parietal cells and partially promoting pepsinogen release. Furthermore, it contributes to positive chronotropic effects in the heart and modulates cytokine secretion in immune cells.
This receptor is a classic therapeutic target for peptic ulcers and gastroesophageal reflux disease, with H2 receptor antagonists (e.g., ranitidine, famotidine) effectively inhibiting excessive acid secretion by blocking this pathway.