CHO-K1 Human D4 Stable Cell

CHO-K1/Human D4 Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0085A	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

The dopamine D4 receptor is a member of the D2-like receptor family, notable for its high genetic polymorphism, which is linked to individual differences. It is primarily distributed in limbic system regions associated with higher cognition and emotion processing, such as the prefrontal cortex, amygdala, and hippocampus. Functionally, the D4 receptor is involved in regulating attention, cognitive flexibility, executive function, and emotional responses. Its genetic variations are a significant risk factor for Attention-Deficit/Hyperactivity Disorder (ADHD) and are also associated with susceptibility to schizophrenia and response to atypical antipsychotic drugs, making it a key target in neuropsychiatric drug development.

Screeningbio’s CHO-K1/Human D4 cell line overexpress DRD4 and is designed to detect inhibition in intracellular cAMP levels in response to agonist stimulation of the receptor. Cisbio HTRF cAMP kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Human
HGNC Symbol	DRD4
Accession Number	NM_000797
Parental Line	CHO-K1
Lot#	See Vial
Storage	Liquid Nitrogen

Data

CHO-K1/Human D4 Agonist Assay. CHO-K1/Human D4 cells were stimulated with 2.5 μM Forskolin and treated with reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software. — **CHO-K1/Human D4 Agonist Assay.** CHO-K1/Human D4 cells were stimulated with 2.5 μM Forskolin and treated with reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software.

Target Background

The dopamine D4 receptor is a member of the D2-like receptor family, notable for its high genetic polymorphism, which is linked to individual differences. It is primarily distributed in limbic system regions associated with higher cognition and emotion processing, such as the prefrontal cortex, amygdala, and hippocampus.

Functionally, the D4 receptor is involved in regulating attention, cognitive flexibility, executive function, and emotional responses. Its genetic variations are a significant risk factor for Attention-Deficit/Hyperactivity Disorder (ADHD) and are also associated with susceptibility to schizophrenia and response to atypical antipsychotic drugs, making it a key target in neuropsychiatric drug development.