Product Description
MET (Mesenchymal-epithelial transition factor receptor), also known as c-Met or HGFR (hepatocyte growth factor receptor) or CD229, is a member of the receptor tyrosine kinase (RTK) family that plays a pivotal role in embryogenesis, tissue regeneration, wound healing, and epithelial-mesenchymal transition (EMT), as well as in cell scattering, proliferation, survival, and morphogenesis. MET is activated by various stimuli including its cognate ligand HGF (hepatocyte growth factor) and cellular stress, leading to downstream activation of the PI3K/AKT, RAS/MAPK, STAT3, and Src/FAK pathways, thereby promoting cell migration, invasion, and resistance to apoptosis. Beyond its role in normal development and tissue repair, dysregulation of MET signaling—through gene amplification, overexpression, activating mutations, or autocrine/paracrine ligand stimulation—is closely associated with multiple cancers (including non-small cell lung cancer, gastric cancer, renal cell carcinoma, hepatocellular carcinoma, and glioblastoma), as well as with resistance to targeted therapies (e.g., EGFR inhibitors), making it a highly validated target for cancer therapy (including MET-specific tyrosine kinase inhibitors and monoclonal antibodies) and drug discovery.
Screeningbio’s MET Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying MET kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: MET catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the MET kinase.
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