Product Description
MARK1 (Microtubule affinity-regulating kinase 1), also known as PAR-1c or MARK, is a member of the serine/threonine protein kinase family (MARK/Par-1 subfamily) that plays a pivotal role in microtubule dynamics, cell polarity, neuronal development, and intracellular transport, as well as in cell cycle regulation and cell migration. MARK1 is activated by various stimuli including LKB1-mediated phosphorylation, calcium signals, and cellular stress, leading to phosphorylation of microtubule-associated proteins (MAPs) such as Tau, MAP2, and MAP4, thereby causing microtubule destabilization and facilitating cytoskeletal reorganization. Beyond its role in normal neuronal architecture and epithelial polarity, dysregulation of MARK1 signaling—through overexpression, hyperactivation, or genetic mutations—is closely associated with neurodegenerative diseases (including Alzheimer's disease and tauopathies), neurodevelopmental disorders (e.g., autism spectrum disorder), and multiple cancers (such as breast cancer and glioblastoma), making it a highly validated target for neuroprotective therapy and cancer drug discovery.
Screeningbio’s MARK1 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying MARK1 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: MARK1 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the MARK1 kinase.
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