Product Description
JAK3 (Janus kinase 3), also known as JAKL, is a member of the non-receptor tyrosine kinase family (Janus kinase subfamily) that plays a pivotal role in cytokine receptor signaling via the common gamma chain (γc), including IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21 receptors, as well as in lymphocyte development, T-cell and NK-cell homeostasis, and immune responses. JAK3 is activated by various stimuli including cytokine engagement, leading to phosphorylation of STAT transcription factors (primarily STAT5, STAT3, and STAT6), thereby promoting cell survival, proliferation, and differentiation. Beyond its role in normal lymphoid development and immune function, dysregulation of JAK3 signaling—through loss-of-function mutations (leading to severe combined immunodeficiency, SCID) or gain-of-function mutations (associated with autoimmune diseases and lymphoproliferation)—is closely associated with primary immunodeficiencies, rheumatoid arthritis, ulcerative colitis, and multiple cancers (including T-cell acute lymphoblastic leukemia and natural killer/T-cell lymphoma), making it a highly validated target for immunosuppressive therapy, autoimmune disease treatment, and cancer drug discovery.
Screeningbio’s JAK3 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying JAK3 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: JAK3 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of theJAK3 kinase.
Data