Product Description
ITK (Interleukin-2-inducible T-cell kinase), also known as EMT or TSK, is a member of the non-receptor tyrosine kinase family (Tec kinase subfamily) that plays a pivotal role in T-cell receptor (TCR) signaling, Th2 differentiation, and immune responses. ITK is activated by various stimuli including TCR engagement and co-stimulatory signals, leading to phosphorylation of PLCγ1, calcium influx, and activation of NF-AT, NF-κB, and MAPK pathways, thereby promoting T-cell activation, proliferation, and cytokine production. Beyond its role in normal adaptive immunity, dysregulation of ITK signaling—through loss-of-function mutations or overexpression—is closely associated with primary immunodeficiency (e.g., X-linked lymphoproliferative disease), allergic asthma, and multiple cancers (including T-cell leukemia and lymphoma), making it a highly validated target for immunosuppressive therapy, asthma treatment, and cancer drug discovery.
Screeningbio’s ITK Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying ITK kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: ITK catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the ITK kinase.
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