Product Description
IKKα (Inhibitor of nuclear factor kappa-B kinase subunit alpha), also known as CHUK, is a member of the serine/threonine protein kinase family (IκB kinase subfamily) that plays a pivotal role in the alternative NF-κB signaling pathway, epidermal development, and keratinocyte differentiation. IKKα is activated by various stimuli including TNFα and developmental cues, leading to processing of p100 to p52 and modulation of both canonical and non-canonical NF-κB activity. Beyond its role in immune regulation and skin homeostasis, dysregulation of IKKα signaling—through loss-of-function mutations or aberrant expression—is closely associated with skin disorders, chronic inflammation, autoimmune diseases, and multiple cancers (including breast cancer and multiple myeloma), making it a highly validated target for anti-inflammatory therapy and cancer drug discovery.
Screeningbio’s IGF1R Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying IGF1R kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: IKKα catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the IKKα kinase.
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