Product Description
FES (Feline sarcoma oncogene), also known as FPS (Fujinami poultry sarcoma oncogene), is a member of the non-receptor tyrosine kinase family that plays a pivotal role in cell proliferation, differentiation, survival, migration, and inflammatory signaling. FES is activated by autophosphorylation in response to various stimuli including growth factors (e.g., GM-CSF, IL-3, EGF), cytokines, and integrin-mediated adhesion, leading to downstream activation of pathways such as PI3K-AKT, STATs, and MAPK. Beyond its role in normal hematopoiesis, neuronal development, and innate immunity, dysregulation of FES signaling—through mutations, altered expression, or aberrant activation—is closely associated with leukemia, colorectal cancer, breast cancer, inflammatory diseases, and impaired wound healing, making it a potential target for cancer therapy and drug discovery.
Screeningbio’s FES Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying FES kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: FES catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the FES kinase.
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