Product Description
ERK2 (Extracellular signal-regulated kinase 2), also known as MAPK1 (mitogen-activated protein kinase 1), is a member of the serine/threonine protein kinase family that plays a pivotal role in the Ras-Raf-MEK-ERK signaling cascade regulating cell proliferation, differentiation, survival, and gene expression. ERK2 is activated by phosphorylation via MEK1/2 in response to diverse extracellular stimuli including growth factors (e.g., EGF, NGF, PDGF), hormones, and cytokines, which transduce signals from receptor tyrosine kinases and other cell surface receptors. Beyond its role in normal cellular physiology, dysregulation of ERK2 signaling—frequently through hyperactivation or mutations in upstream pathways (e.g., KRAS, BRAF)—is closely associated with tumorigenesis, metastatic progression, developmental disorders (such as Noonan syndrome), inflammatory diseases, and drug resistance, making it a highly validated target for cancer therapy and drug discovery.
Screeningbio’s ERK2 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying ERK2 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: ERK2 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the ERK2 kinase.
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