Product Description
EIF2AK2 (Eukaryotic Translation Initiation Factor 2 Alpha Kinase 2), also known as PKR (dsRNA-dependent protein kinase), is a member of the serine/threonine protein kinase family that plays a pivotal role in innate immunity, antiviral defense, and cellular stress responses. EIF2AK2 is activated by double-stranded RNA, interferon, oxidative stress, and endogenous non-coding RNAs, leading to phosphorylation of the eukaryotic translation initiation factor eIF2α, which globally inhibits protein synthesis to restrict viral replication and modulate autophagy, apoptosis, and cell survival. Beyond its antiviral function, dysregulation of EIF2AK2 signaling is closely associated with neurodegenerative diseases (such as Alzheimer's disease), metabolic syndrome, ischemic injury, and multiple malignancies (including glioblastoma, breast cancer, and hepatocellular carcinoma), making it a highly validated target for antiviral therapy, cancer treatment, and neuroprotective drug discovery.
Screeningbio’s EIF2AK2 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying EIF2AK2 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: EIF2AK2 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the EIF2AK2 kinase.
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