Product Description
DNA-PK (DNA-dependent protein kinase) is a serine/threonine kinase complex composed of the catalytic subunit DNA-PKcs (DNA-PK catalytic subunit) and the Ku70/Ku80 heterodimer, which plays a pivotal role in DNA double-strand break repair, particularly through the non-homologous end joining (NHEJ) pathway, as well as in V(D)J recombination, telomere maintenance, and transcriptional regulation. DNA-PK is activated upon binding to DNA ends, leading to autophosphorylation and phosphorylation of downstream substrates involved in repair complex assembly and cell cycle checkpoint control. Beyond its essential physiological functions in maintaining genomic stability and immune system development, dysregulation of DNA-PK signaling—including overexpression, hyperactivation, and aberrant repair activity—is closely associated with various human cancers, contributing to tumor resistance to radiotherapy and DNA-damaging chemotherapies, as well as with neurodegenerative disorders and autoimmune diseases. These features have established DNA-PK as a highly validated target for cancer therapy, particularly in combination with DNA-damaging agents and as a strategy to overcome treatment resistance.
Screeningbio’s DNA-PK Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying DNA-PK kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: DNA-PK catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the DNA-PK kinase.
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