Product Description
DAPK1 (Death-associated protein kinase 1) is a member of the serine/threonine kinase family that plays a pivotal role in apoptosis, autophagy, and cytoskeletal dynamics. DAPK1 is activated by calcium/calmodulin binding and through autophosphorylation, and functions as a key mediator of cell death signaling in response to various cellular stresses. Beyond its essential physiological functions in tumor suppression and tissue homeostasis, dysregulation of DAPK1 signaling—including loss-of-function mutations, promoter hypermethylation, and altered expression—is closely associated with various human cancers (such as lung, breast, and colorectal cancer), as well as with neurodegenerative diseases (including Alzheimer's disease, Parkinson's disease, and cerebral ischemia), making it a highly validated target for cancer therapy and neuroprotective drug discovery.
Screeningbio’s DAPK1 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying DAPK1 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: DAPK1 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the DAPK1 kinase.
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