Product Description
CAMK2α (Calcium/calmodulin-dependent protein kinase II alpha), also known as CaMKIIα, is a member of the serine/threonine kinase family that plays a pivotal role in calcium signaling, synaptic plasticity, learning, and memory formation. CAMK2α is activated by calcium-bound calmodulin, leading to autophosphorylation and sustained activity, and subsequently phosphorylates a wide range of downstream substrates involved in neuronal excitability, cytoskeletal dynamics, and gene transcription. Beyond its essential physiological functions in the central nervous system, dysregulation of CAMK2α signaling—including aberrant expression, overactivation, or genetic mutations—is closely associated with neurodevelopmental disorders (such as autism spectrum disorder and intellectual disability), neurodegenerative diseases (including Alzheimer's disease), cardiac arrhythmias, and ischemia/reperfusion injury, making it a highly validated target for therapeutic intervention in neurology and cardiology.
Screeningbio’s CAMK2α Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying CAMK2α kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: CAMK2α catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the CAMK2α kinase.
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