Product Description
CAMK1γ (Calcium/calmodulin-dependent protein kinase type 1 gamma), also known as CaMKIγ, is a member of the serine/threonine kinase family that plays a pivotal role in calcium signaling, neuronal development, cell migration, and gene expression regulation. CAMK1γ is activated by calcium-bound calmodulin and subsequent phosphorylation by upstream kinases such as CaMKK, leading to phosphorylation of downstream substrates involved in transcription and cytoskeletal dynamics. Beyond its essential physiological functions in the nervous system and immune responses, dysregulation of CAMK1γ signaling—including aberrant expression and altered activity—has been implicated in various human diseases, such as neurodevelopmental disorders, inflammatory conditions, and cancer progression, making it a highly validated target for therapeutic intervention.
Screeningbio’s CAMK1γ Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying CAMK1γ kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: CAMK1γ catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the CAMK1γ kinase.
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