Product Description
ALK6 (Activin Receptor-Like Kinase 6), also known as BMPR1B (Bone Morphogenetic Protein Receptor Type 1B), is a member of the serine/threonine kinase family and functions as a type I receptor for bone morphogenetic proteins (BMPs), playing a pivotal role in skeletal development, chondrogenesis, and reproductive function. ALK6 transduces signals upon ligand binding by forming complexes with type II BMP receptors, leading to phosphorylation of downstream SMAD transcription factors. Beyond its essential physiological functions, dysregulation of ALK6 signaling—including loss-of-function mutations and aberrant expression—has been closely associated with brachydactyly type C, acromesomelic dysplasia, and various cancers, such as colorectal and breast cancer, making it a highly validated target for therapeutic intervention in skeletal disorders and oncology.
Screeningbio’s ALK6 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying ALK6 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: ALK6 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the ALK6 kinase.
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