OVCAR5/TCF-LEF/Luciferase Reporter Cell Line
Item | Cat# | Price |
Stable Cell Line | SNB-SP-0004 | $19,80 |
Compound Test Services | CT-001 | $1,850 per 384w plate (Up To 16 cpds Dose) |
Product Description
The Wnt(TCF/LEF) signaling pathway, or the canonical Wnt/β-catenin pathway, hinges on the regulated stability of β-catenin protein. In the absence of Wnt signaling, β-catenin is constitutively degraded by a destruction complex. Upon Wnt ligand binding to its membrane receptors, β-catenin is stabilized, accumulates, and translocates into the nucleus. There, it acts as a co-activator by binding to TCF/LEF transcription factors to initiate the expression of key genes (e.g., c-Myc) governing embryonic development and cell proliferation. Dysregulation of this pathway is closely linked to cancer.
Screeningbio’s OVCAR5/TCF-LEF/Luciferase reporter gene cell line stable transfected with TCF-LEF response element and luciferase gene. Upon stimulated, TCF-LEF pathway was activated and induce luciferase expression.
Product Specifications
Target Type | Signal Pathway |
Species | Human |
HGNC Symbol | |
Accession Number | |
Parental Line | OVCAR5 |
Lot# | See Vial |
Storage | Liquid Nitrogen |
Data
Target Background
The Wnt(TCF/LEF) signaling pathway, or the canonical Wnt/β-catenin pathway, hinges on the regulated stability of β-catenin protein. In the absence of Wnt signaling, β-catenin is constitutively degraded by a destruction complex. Upon Wnt ligand binding to its membrane receptors, β-catenin is stabilized, accumulates, and translocates into the nucleus.
There, it acts as a co-activator by binding to TCF/LEF transcription factors to initiate the expression of key genes (e.g., c-Myc) governing embryonic development and cell proliferation. Dysregulation of this pathway is closely linked to cancer.